WebTreatment with 5-Aza-CdR significantly promoted the MMC chemosensitivity of T24 cells. The proliferation of T24 cells was significantly inhibited with increasing 5-Aza-CdR concentration, whereas apoptosis was significantly increased. This was associated with the decreased expression of P-gp, MRP1, beclin 1, p62 and ATG5. WebAnti-APG5L/ATG5 antibody [EPR1755(2)] Abcam catalog: ab108327 total number of products: 9. domestic rabbit monoclonal (EPR1755(2)) reactivity: human, mouse, rat …
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WebApr 12, 2024 · Lysosomal homeostasis and membrane maintenance involve ESCRT-dependent mechanisms, with protein ALIX playing a major role in lysosomal membrane … WebNov 1, 2014 · Here, Atg5 knockdown decreased the expression of LC3-II and P62, which indicated that LNs lead to autophagic response in neuron cells via an Atg5-dependent way. We postulate that autophagy is an Atg5-dependent host-defense mechanism, which is an active response to the cytotoxicity of the nanocarriers in cells.41, 42, 43, 44
WebApr 11, 2024 · Atg5, Atg7, beclin1, LC3B, FOXO1, FOXO3a, FOXO4, and FOXO6 genes were evaluated using real-time PCR. Atg5, beclin1, LC3B, and FOXO1 expression at the protein level was evaluated by the western blot technique. ... (National Institutes of Health). All antibodies were purchased from the Abcam company (Abcam, USA). Statistical … WebAll lanes : Anti-APG5L/ATG5 antibody (ab228668) at 1/1000 dilution Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 50 µM chloroquine for 24 hours, whole cell extracts Lysates/proteins at 30 µg per lane. …
WebAtg5 -/- MEFs were kindly provided by Dr. Ramnik Xavier, Massachusetts General Hospital, Harvard Medical School, Boston, MA. Immunoprecipitation of Atg5 from PANC-1 cell … Web自噬交互通路有助于探索蛋白质在起始、成核、延伸、成熟、融合和降解等各个阶段的相互作用。阅读弹出窗口和包含 Abcam 全部产品的产品列表，轻松找到最适合您目标靶点 …
WebMar 31, 2024 · Moreover, silencing of ATG5 in CAFs inhibited fibroblasts autophagic level and suppressed malignant phenotypes of PCa cells, while ATG5 overexpression in NFs exerted opposite effects. ... (Abcam), anti-LC3 I/II (Invitrogen, Carlsbad, CA, USA), anti-p62 (Abcam), anti-β-tubulin (Abcam) and anti-ATG5 (Abcam). Then membranes were …
WebDec 16, 2024 · Autophagy has been described to be both protective and pathogenic in cerebral ischemia/reperfusion (I/R) injury. The underlying association between autophagy and ferroptosis in ischemic stroke has not yet been clearly investigated. The purpose of this study was to explore the role of autophagy-related gene 5 (ATG5) in experimental … can you keep a pet owl in australiaWebSep 3, 2024 · This is a Validated Antibody Database (VAD) review about human ATG5, based on 133 published articles (read how Labome selects the articles), using ATG5 … can you keep an orb weaver as a petWebLane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: APG5L/ATG5 knockout HAP1 cell lysate (20 µg) Lane 3: Raji cell lysate (20 µg) Lane 4: Jeg-3 cell lysate (20 µg). Lanes 1 - 4: … bright star shopping cartWebJan 22, 2024 · The primary rabbit antibodies used were as follows: TSG101 (1:1000, Abcam, ab125011), HSP70 (1:1000, Abcam, ab2787), ATG5 (1:1000, Abcam, ab228668), and … can you keep an owl as a pet ukWebKnockout Tested Rabbit recombinant monoclonal APG5L/ATG5 antibody [EPR1755(2)]. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat, Human. ... The reviewer … can you keep a northern mockingbird as a petWebMay 31, 2016 · We first investigated whether knockout of Atg5 or Atg7 in immortalized mouse embryonic fibroblasts (MEFs) regulates ferroptosis. ... (Abcam, ab83366 and ab118970) (n = 3; *, p < 0.05 versus WT group). (E) MEFs and PANC1 cells were treated with erastin “Era” in the presence or absence of CQ for 24 h. can you keep a newt as a petWebApr 12, 2024 · Lysosomal homeostasis and membrane maintenance involve ESCRT-dependent mechanisms, with protein ALIX playing a major role in lysosomal membrane repair. 44, 47, 77, 78 Thus, we tested whether ATG5 has an effect on ALIX recruitment to damaged lysosomes. In Huh7 ATG5-WT cells, ALIX puncta were induced and ALIX co … brightstar shrewsbury