Cy3 dye excitation
Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … WebDyes Click to view the Fluorophores Modifications category Choose how you want to view the dyes: Sorted by Category Sorted by Excitation Sorted by Emission Freedom™ Dyes …
Cy3 dye excitation
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WebCy3 dye. Fluorescence SpectraViewer. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC … WebTexas Red (and Texas Red-X) Dye. Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Texas Red sulfonyl chloride is susceptible to hydrolysis and its low solubility in water may complicate ...
WebFluorescent conjugates. The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.These conjugates are ideal for imaging and other applications requiring increased sensitivity and environmentally … WebCy3.5 dyes have enhanced fluorescence upon binding to proteins. The excitation peak and emission peak of Cy3.5 dye is around 550nm and 590nm respectively. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. ... Cyanine dye should be dissolved in organic solvent and then ...
WebCyDye Fluorescent Dyes carry only one reactive group on each dye molecule for accurate labeling of amine functional groups, for example on antibodies or oligonucleotides. … WebJun 18, 2024 · The second column in the Multiplex Dye Selection Tool offers a list of alternative dyes that are compatible but require instrument calibration (Figure 1, orange …
WebDownload scientific diagram Two Cy3 dye emission spectra. Dashed curve: monochromator scan of the dye excited by a laser. Solid curve: excitation and detection by two LIFs with our EEF.
WebAdd products for $400 to your cart and get free express shipping . Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 ... switch wrath animatronic fnafWebA tandem dye consists of a donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two. The tandem dye is excited at … switch wraps incWebCy3 550 570 yellow TRITC 547 572 yellow Alexa fluor 546 556 573 yellow Alexa fluor 555 556 573 yellow R-phycoerythrin (PE) 480;565 578 yellow ... Dye Absorbance Wavelength Emission Wavelength Visible color DAPI 345 455 blue Hoechst 33258 345 478 blue SYTOX blue 431 480 blue Hoechst 33342 343 483 blue switch wrapsWebEquimolar concentrations of His-tagged W2A cadherin mono- As described previously (Zhang et al., 2009), the cadherin sequences were mers labeled with Cy3 dye and biotinylated W2A cadherin monomers transfected into HEK293 cells and the protein was purified from the condi- labeled with Alexa-647 dye were concentrated to 80 mM and … switchwrc8WebDyLight 550 provides vibrant orange-to-red fluorescence with better performance than other rhodamine derivatives, including Alexa Fluor™ 555, TRITC, and Cy3™ dye for fluorescent applications. The high water … switchwrc9WebCy3.5™ 598nm: 581nm: Cy3™ ... 49037 - ET - Li_Cor for IR dye 800: 49052 - ET - ECFP/EYFP: 49053 - ET - DAPI for 355-405nm LEDs: 49054 - ET - GFP for 455-465nm LEDs: ... ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation: TRF69901 - ET - 405/488/561nm Laser Triple Band Set for TIRF … switch wrap up 2021WebIndocarbocyanine, Cy™3 Technical Information. Cy™3 is brighter, more photostable and gives less background than other orange-red fluorescing dye conjugates. Cy™3 conjugates can be excited maximally at 550 nm, … switch wqhd